TDO2 (NM_005651) Human Recombinant Protein PH40688M5 from Leading Biology

Product Specs
ItemTDO2 (NM_005651) Human Recombinant Protein
CompanyLeading Biology
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Catalog NumberPH40688M5
FormatLiquid
Conjugate/TagC-Myc/DDK
Concentration>50 ug/mL as determined by microplate BCA method
Quantity20 ug
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
NCBI Full Gene Nametryptophan 2,3-dioxygenase
NCBI Gene AliasesHYPTRP, TPH2, TDO, TRPO, TO
Research AreaMetabolic pathways, Tryptophan metabolism
Molecule NameTDO2
TypeRecombinant Proteins
Source/Expression SystemHEK293T
Molecular Weight47.7 kDa
SpeciesHuman
ActivityThe specific activity of TDO2 was determined by monitoring Kynurenine formation from the N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with TDO2. The reactions were carried out at 37°C for 40min in 100ul of the reaction volume containing 200mM PBS, pH7.5, 1mM ascorbic acid, and 1.25mM L-tryptophan as the substrate with various amounts of TDO2. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich's reagent (2% p-dimethylaminobenza-ldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation